Fig. 2: MPT0G211 significantly inhibited tau phosphorylation. | Cell Death & Disease

Fig. 2: MPT0G211 significantly inhibited tau phosphorylation.

From: The novel histone de acetylase 6 inhibitor, MPT0G211, ameliorates tau phosphorylation and cognitive deficits in an Alzheimer’s disease model

Fig. 2

a pRK5-EGFP-Tau P301L was used to transfect SH-SY5Y or Neuro-2a cells for 24 h; then cells were photographed by fluorescence microscopy. Images represented magnification at ×100. b Cells were transfected with pCAX APP 695 for 24 h, harvested, and cell lysates were subjected to Western blotting. c, d SH-SY5Y and Neuro-2a cells were transfected with pCAX APP 695 and/or pRK5-EGFP-Tau P301L for 24 h. Cell lysates were prepared for western blot analysis of the indicated proteins. e, f Cells were transfected with pCAX APP 695 and pRK5-EGFP-Tau P301L for 24 h and were then incubated with or without MPT0G211 or ACY-1215 (0.1 μM) for another 24 h. Cell lysates were subjected to western blot analysis using the indicated antibodies. Results are presented as the mean ± SEM from three independent experiments. **p < 0.01, ***p < 0.001 compared with the control group; #p < 0.05, ##p < 0.01 compared with the indicated groups

Back to article page