Fig. 3: Wnt genes were differentially expressed in myofibroblast differentiation of lung-resident mesenchymal stem cells (LR-MSCs) and fibrotic lung tissues.

a, b Quantitative PCR analysis showed the relative mRNA levels of Wnt genes in TGF-β1-treated LR-MSCs (a) and bleomycin (BLM)-induced fibrotic lung tissue (b). c Levels of Wnt7b and Wnt10a in LR-MSCs treated with TGF-β1 were measured by western blot. d, e The expression of Wnt7b and Wnt10a in BLM-induced mouse fibrotic lung tissues was determined by western blot (d) and immunohistochemistry (e). f Immunodetection showed Wnt7b and Wnt10a in human normal (Con) lung tissues (left panel) and IPF lung tissues (right panel). Data represented three independent experiments