Fig. 2: ARHGAP15 overexpression suppressed the proliferation, migration, and invasion and induced G1 phase arrest of CRC cells.

HT29 and RKO cells were transfected with pLVX-ARHGAP15 or pLVX-NC. a Cell proliferation detected by CCK-8 assay. b Cell cycle distribution analyzed by flow cytometry. c Cell migration detected by transwell assay. Scale bar: 100 μm. d Cell invasion detected by transwell assay. Scale bar: 100 μm. Experiments were repeated three times independently. NS: no significant difference; *** P < 0.001