Fig. 1: Zfp90 is essential for the maintenance of HSPC pools. | Cell Death & Disease

Fig. 1: Zfp90 is essential for the maintenance of HSPC pools.

From: The transcription factor Zfp90 regulates the self-renewal and differentiation of hematopoietic stem cells

Fig. 1

a Expression profiles of transcription factors (TFs) in HSCs and MPPs were analyzed using R language and Bioconductor according to Jun Seita’s cohort (GSE34723). b Total RNA was extracted from representative hematopoietic populations. Expression levels of Zfp90 were analyzed by real-time qPCR. Fold changes were normalized to endogenous Actb. c Expression levels of Zfp90 in indicative GFP+CD45.2+BM cells were evaluated by WB. β-actin was used to indicate the amount of loading proteins. d Analysis of whole CD45.2+GFP+ donor cell-derived bone marrow cells (BMC) in indicative chimera mice 16 weeks after transplantation. n = 6 for each group. e Analysis of long-term hematopoietic stem cells (LT-HSC), short-term hematopoietic stem cells (ST-HSC), and multipotential hemopoietic progenitors (MPP) in Zfp90+/+ and Zfp90−/− mice 16 weeks after transplantation. n = 6 for each group. f Analysis of common lymphoid progenitors (CLP) in Zfp90+/+ and Zfp90−/− mice 16 weeks after transplantation. CLP is gating on Lin−CD127+c-Kit+Sca1+. n = 6 for each group. g Analysis of common myeloid progenitors (CMP), granulocyte-macrophage progenitors (GMP) and megakaryocyte-erythroid progenitors (MEP) in Zfp90+/+ and Zfp90−/− mice 16 weeks after transplantation. n = 6 for each group. h Numbers of representative hematopoietic progenitor cells in Zfp90+/+ and Zfp90−/− mice were determined 16 weeks after transplantation. n = 6 for each group. i Peripheral blood cell counts were obtained from Zfp90-deleted and control mice 16 weeks after transplantation. Lymphocytes and granulocytes were analyzed. n = 6 for each group. j The numbers of HSCs, myeloid cells and neutrophils were calculated by FACS in the spleen from Zfp90+/+ and Zfp90−/− mice 16 weeks after transplantation. k GMP/MEP ratio and the CMP/CLP ratio in Zfp90−/− vs Zfp90+/+ mice were analyzed via FACS. *p<0.05 and **p<0.01 by two-tailed Student’s t test. All data presented are shown as the means ± SD collected from three independent experiments

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