Fig. 3: Zfp90-mediated HSC loss is cell intrinsic. | Cell Death & Disease

Fig. 3: Zfp90-mediated HSC loss is cell intrinsic.

From: The transcription factor Zfp90 regulates the self-renewal and differentiation of hematopoietic stem cells

Fig. 3

a Schematic representation for BM transplantation assays. b Analysis of LT-HSCs, ST-HSCs, MPPs and myeloid progenitors (MP) in chimeras reconstituted with Zfp90+/+ or Zfp90−/− BM cells. n = 6 for each group. c Analysis of CLPs in chimeras reconstituted with Zfp90+/+ or Zfp90−/− BM cells. n = 6 for each group. d Enumeration of donor-derived HSCs, MPPs, CLPs and MPs in chimeras. n = 6 for each group. e Analysis of donor-derived CD45.2+ T, B, NK and Gran cells in the chimera blood. n = 6 for each group. f Flow cytometric analysis of the indicated cells from competitive BM chimera. Ratios of LT-HSCs, ST-HSCs and MPPs in chimeras were analyzed. *p<0.05, **p<0.01, and ***p<0.001 by two-tailed Student’s t test. All data presented are shown as the means±SD collected from three independent experiments

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