Fig. 5: Zfp90 cooperates with the NURF complex to regulate Hoxa9 expression. | Cell Death & Disease

Fig. 5: Zfp90 cooperates with the NURF complex to regulate Hoxa9 expression.

From: The transcription factor Zfp90 regulates the self-renewal and differentiation of hematopoietic stem cells

Fig. 5

a Relative expression of representative HSC-proliferation-related genes. HSCs were isolated from Zfp90+/+ and Zfp90−/− mice 16 weeks after transplantation. b–d Analysis of representative gene expression in Bptf-, Snf2l- or Rbbp4-deleted HSCs and WT control. Two sgRNAs were used for the deletion of Bptf, Snf2l or Rbbp4. e Analysis of Zfp90 enrichment on Hoxa9 promoter in LSK (Lin−Sca-1+c-Kit+) cells via ChIP assays. f Analysis of the direct interaction of Zfp90 with Hoxa9 promoter via EMSA assays. g Myc-Zfp90, pTK, and pGL3-Hoxa9 WT (region: −2000 to 0) or Mutant (Mut region: deletion of −550 to −800) promoter were transfected into 293T cells for luciferase assay. h, i Analysis of Bptf or Snf2l enrichment on Hoxa9 promoter in Zfp90+/+ or Zfp90−/− LSKs. j Accessibility of Hoxa9 promoter to DNase I in Zfp90+/+ or Zfp90−/− LSKs was assessed. *p<0.05, **p<0.01, and ***p<0.001 by two-tailed Student’s t test. All data presented are shown as the means±SD collected from three independent experiments

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