Fig. 6: The PKA–CREB axis acts as the downstream of CTRP9–CRT interaction in MI/R injury. | Cell Death & Disease

Fig. 6: The PKA–CREB axis acts as the downstream of CTRP9–CRT interaction in MI/R injury.

From: Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis

Fig. 6: The PKA–CREB axis acts as the downstream of CTRP9–CRT interaction in MI/R injury.

a, b Western blot analysis of PKA, AMPK, ERK1/2, and Akt phosphorylation in vivo. c H89 administration inhibits PKA activation in vitro. d Effect of PKA inhibition upon CREB phosphorylation. e, f Effect of PKA inhibition upon LDH release and caspase-3 activity. g–i Effect of CRT or AdipoR1 deficiency on PKA and CREB activation. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 vs. control group; $P < 0.05, $$P < 0.01 between the two groups connected by line. N = 3–6

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