Fig. 6: The functional role of miR-514b-3p and miR-514b-5p in the EMT progression of CRC cell lines.
From: The distinct role of strand-specific miR-514b-3p and miR-514b-5p in colorectal cancer metastasis
a–d GSEA was performed to identify the difference of gene profiles in control-miRNA group, miR-514b-3p overexpression group and miR-514b-5p overexpression group. e, f Real-time PCR was performed to measure the mRNA expression of EMT markers (E-cadherin, CLDN-1, fibronectin-1 and vimentin) in LOVO and HCT116 after transfection of miR-514b-3p or miR-514b-5p mimics. Error bars represented SEM. g, h Western-blot was conducted to evaluate the protein expression of EMT markers (E-cadherin, CLDN-1, fibronectin-1, and vimentin) in LOVO and HCT116 after transfection of miR-514b-3p or miR-514b-5p mimics. GAPDH was used as an internal control. i Schematic representation for the mechanism of miR-514 regulates EMT in human CRC progression
