Fig. 1: Morphology and purity of the cell lines, and PCR detection for HPV and mycoplasma.

a Phase-contrast photomicrograph of cell lines. b Flow cytometry assay for Pan-CK expression, indicating the purity of new cell lines. c Western blotting (WB) assay for the expression of Pan-CK and Vimentin (cancer-associated fibroblast (CAF) was used as a control). d PCR detection of mycoplasma contamination in cell lines (+, positive control)