Fig. 5: LINC00324 can bind with HuR. | Cell Death & Disease

Fig. 5: LINC00324 can bind with HuR.

From: Long intergenic non-coding RNA 00324 promotes gastric cancer cell proliferation via binding with HuR and stabilizing FAM83B expression

Fig. 5

a, b The expression levels of LINC00324 in the cytoplasm or nucleus of BGC823 and SGC7901 cells were detected by qRT-PCR. GAPDH was used as a cytosol marker and U6 was used as a nuclear marker. c The prediction of the interaction probabilities of LINC00324 with RNA-binding protein HuR by Bioinformatics (http://pridb.gdcb.iastate.edu/RPISeq/). Predictions with probabilities >0.5 were considered “positive”, indicating that the corresponding RNA and protein are likely to interact. d, e RNA immunoprecipitation experiments were performed in BGC823 and SGC7901 cells and the coprecipitated RNA was subjected to qRT-PCR for LINC00324. The fold enrichment of LINC00324 in HuR RIP is relative to its matching IgG control. Values are shown as the mean ± standard errors of the mean based on three independent experiments. *P < 0.05, **P < 0.01

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