Fig. 7: Increased expression of FAM83B promotes GC cell proliferation and is involved in the oncogene function of LINC00324. | Cell Death & Disease

Fig. 7: Increased expression of FAM83B promotes GC cell proliferation and is involved in the oncogene function of LINC00324.

From: Long intergenic non-coding RNA 00324 promotes gastric cancer cell proliferation via binding with HuR and stabilizing FAM83B expression

Fig. 7

a Relative expression of FAM83B in 66 paired human GC tissues and their corresponding adjacent non-tumor tissues were analyzed by qRT-PCR and normalized against GAPDH expression. The data are presented as the delta CT value. b qRT-PCR analysis of FAM83B expression in normal gastric epithelium cell line (GES1) and GC cells. c qRT-PCR analysis of FAM83B expression in BGC823 and SGC7901 cells transfected with control (scrambled), si-FAM83B 1#, si-FAM83B 2# and si-FAM83B 3#. d, e MTT and colony-formation assays were performed to determine the viability of BGC823 and SGC7901 cells transfected with si-FAM83B 3# or control (scrambled). Experiments were performed in triplicate. f Migration ability was investigated by transwell assays in BGC823 and SGC7901 cells transfected with si-FAM83B 3# or control (scrambled). g, h MTT and colony-formation assays were used to determine the cell viability of si-FAM83B 3# and pcDNA-LINC00324 co-transfected BGC823 and SGC7901 cells. Experiments were performed in triplicate. Values are shown as the mean ± standard errors of the mean from three independent experiments. *P < 0.05, **P < 0.01

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