Fig. 2: Protein levels of Hh activators and EMT-related genes in hepatocytes from PH livers | Cell Death & Disease

Fig. 2: Protein levels of Hh activators and EMT-related genes in hepatocytes from PH livers

From: MicroRNA-378 is involved in hedgehog-driven epithelial-to-mesenchymal transition in hepatocytes of regenerating liver

Fig. 2

a Western blot analysis for SMO (86 kDa), nuclear GLI3 (145 kDa) and p65 (65 kDa), processed form of TGFb (25 kDa), VIMENTIN (57 kDa), αSMA (42 kDa), E-cadherin (120 kDa), GAPDH (36 kDa), and LAMINb1 (68 kDa) in primary hepatocytes isolated from PH livers of mice. GAPDH and LAMINb1 was used as an internal control. Immunoblots shown represent one of three independent experiments with similar results. b Cumulative densitometric analyses of SMO, GLI3, p65, TGFb, Vimentin, αSMA, and E-cadherin western blots are displayed as the mean ± s.e.m. (unpaired two-sample Student’s t test, *p < 0.05 **p < 0.005 vs. 0 h)

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