Fig. 3: MiR-378 inhibitor promotes EMT in primary hepatocytes of mice.

a qRT-PCR of miR-378, Gli3, Tgfb, Snail, Col1a1, Vimentin and E-cadherin in primary hepatocytes (pHEP), which were isolated from WT mice and transfected with miR-378 inhibitor (I, 75 nM, diagonal lined bar) or scramble miR (NC, 75 nM, white bar) for 12 and 24 h. All results of relative expression values are shown as mean ± s.e.m. of triplicate experiments (unpaired two-sample Student’s t test, *p < 0.05 **p < 0.005 vs. both cells cultured alone (black bar) and cultured with NC). b Western blot analysis and cumulative densitometric analyses for SMO, VIMENTIN, E-cadherin, and GAPDH in these transfected cells for 24 h. GAPDH was used as an internal control. Immunoblots shown represent one of three independent experiments with similar results. Results are displayed as the mean ± s.e.m. (unpaired two-sample Student’s t test, *p < 0.05 **p < 0.005 vs. both cells cultured alone (black bar) and cultured with NC)