Fig. 8: MiR-378 suppresses hepatocyte EMT in mice with PH. | Cell Death & Disease

Fig. 8: MiR-378 suppresses hepatocyte EMT in mice with PH.

From: MicroRNA-378 is involved in hedgehog-driven epithelial-to-mesenchymal transition in hepatocytes of regenerating liver

Fig. 8

a qRT-PCR for Smo, miR-378, Gli3, p65, Tgfb, Snail, Vimentin, and E-cadherin in hepatocyte which were isolated from PH or quiescent liver and transfected with miR-378 mimic (PH with miR378: diagonal lined bar) or NC (nonPH with NC: white bar/ PH with NC: black bar). All results of relative expression values are shown as mean ± s.e.m. of triplicate experiments (one-way ANOVA with Tukey corrections, *p < 0.05 **p < 0.005 vs. nonPH with NC, $p < 0.05 $$p < 0.005 vs. PH with NC). b Western blot and cumulative densitometric analyses for SMO (86 kDa), GLI3 (145 kDa), VIMENTIN (57 kDa), SNAIL (29 kDa), E-cadherin (120 kDa), and GAPDH (36 kDa) in these cells. GAPDH was used as an internal control. Immunoblots shown represent one of three independent experiments with similar results. Results are displayed as the mean ± s.e.m. (*p < 0.05, **p < 0.005 vs. nonPH with NC, $p < 0.05 $$p < 0.005 vs. PH with NC)

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