Fig. 3: Parkin is recruited into TNF-RSC in RIPK1-dependent manner.
From: Parkin regulates NF-κB by mediating site-specific ubiquitination of RIPK1
a WT and Ripk1−/− MEFs stably expressing Parkin were treated with TNFα 10 ng/mL for 0, 5, and 15 min. The cell lysates were immunoprecipitated with anti-TNFR1. The immunoprecipitates and cell lysates were analyzed by western blotting with indicated antibodies. b 661 W cells stably expressing GFP, Parkin or K150E Parkin were treated with TNFα10 ng/mL for 0, 5, and 15 min. The cell lysates were immunoprecipitated with anti-RIPK1. The immunoprecipitates and cell lysates were analyzed by western blotting with indicated antibodies. c 661 W cells stably expressing GFP, WT Parkin or K150E mutant Parkin were treated with TNFα 10 ng/mL for 0, 5, and 15 min. Complex I was immunoprecipitated with anti-TNFR1. The immunoprecipitates and cell lysates were analyzed by western blotting with indicated antibodies
