Fig. 2: Marked electroretinographic alterations and histological damages are associated with irreversible vision deficits.
From: Nogo-A inactivation improves visual plasticity and recovery after retinal injury
a Representative ERG waveforms recorded at different intensities of light stimulation after PBS and NMDA injection. b Quantitatively, ERG b-wave was significantly reduced after intravitreal injection of 5 nmol of NMDA compared with PBS and the administration of 0.5 nmol of NMDA. ERG a-wave amplitudes did not vary. c Immunofluorescent stainings of RGCs and amacrine cells, respectively, with RBPMS, ChAT, and Calretinin. d–f Quantitative analysis of cell survival. The number of RBPMS-labeled RGCs was affected at 0.5 nmol of NMDA. A unit of 5 nmol of NMDA dramatically reduced the number of cells stained with the three markers. Statistics: one-way ANOVA followed by Tukey post hoc test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bar in c = 100 μm
