Table 1 Primer sequences used for qRT-PCR measurements in mouse retinal lysates after intravitreal injections of NMDA.
From: Nogo-A inactivation improves visual plasticity and recovery after retinal injury
Gene names | Forward (5′–3′) | Reverse (5′–3′) | Product (bp) |
|---|---|---|---|
Cox2 | GACAGATCATAAGCGAGGAC | TACACCTCTCCACCAATGAC | 153 |
Cntf | CTCTGTAGCCGCTCTATCTG | GGTACACCATCCACTGAGTC | 125 |
Gapdh | CAGCAATGCATCCTGCACC | TGGACTGTGGTCATGAGCCC | 96 |
Gap43 | TGCTGTCACTGATGCTGCT | GGCTTCGTCTACAGCGTCTT | 127 |
Gfap | CCACCAAACTGGCTGATGTCTAC | TTCTCTCCAAATCCACACGAGC | 240 |
Il6 | ACCGCTATGAAGTTCCTCTC | CTCCGACTTGTGAAGTGGTA | 163 |
Lif | AATGCCACCTGTGCCATACG | CAACTTGGTCTTCTCTGTCCCG | 216 |
Rtn4 (Nogo-A) | CAGTGGATGAGACCCTTTTTG | GCTGCTCCTTCAAATCCATAA | 90 |
Rtn4r (NgR1) | CTCGACCCCGAAGATGAAG | TGTAGCACACACAAGCACCAG | 116 |
S1pr1 | TCAGGGAACTTTGCGAGTGA | AACAGCAGCCTCGCTCAAG | 123 |
S1pr2 | CATCGCCATCGAGAGACAAG | TCAGACAATTCCAGCCCAGG | 146 |
Sprr1a | GAACCTGCTCTTCTCTGAGT | AGCTGAGGAGGTACAGTG | 91 |
Stat3 | CAAAACCCTCAAGAGCCAAGG | TCACTCACAATGCTTCTCCGC | 139 |
Tnfa | CCACGCTCTTCTGTCTACTGA | GGCCATAGAACTGATGAGAGG | 92 |
Tubb3 | GGCCTCCTCTCACAAGTATG | TTGCCAGCACCACTCTGAC | 138 |
Vim | TACAGGAAGCTGCTGGAAGG | TGGGTGTCAACCAGAGGAA | 113 |
