Fig. 5: SOX17 induces cell cycle arrest by trans-suppressing the Wnt/β-catenin pathway in cervical cancer cells. | Cell Death & Disease

Fig. 5: SOX17 induces cell cycle arrest by trans-suppressing the Wnt/β-catenin pathway in cervical cancer cells.

From: SOX17 restrains proliferation and tumor formation by down-regulating activity of the Wnt/β-catenin signaling pathway via trans-suppressing β-catenin in cervical cancer

Fig. 5

a Visualization of known genes for Wnt/β-catenin pathway in HeLa-GFP cells (right) and HeLa-SOX17 cells (left) as a heatmap using microarray. Data were log2 normalized. Real-time PCR analysis is shown for the mRNA levels of Wnt/β-catenin pathway key genes in SOX17 overexpression and knockdown HeLa (b) and SiHa (c) cells. The expression of Wnt/β-catenin pathway key proteins in SOX17 modified HeLa and SiHa cells were determined by western blot (d, e) and the quantitative analysis (f–i) was shown. j–m SOX17 modified HeLa and SiHa cells were transfected with the TOP/FOP-Flash reporter plasmid, and the reporter activities were detected 48 h after transfection by a luciferase assay. Data represent mean ± SD of triplicate experiments and statistical analysis was done with Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001

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