Fig. 3: Histological analysis of muscle samples of WT, mdx and nintedanib-treated mdx mice. | Cell Death & Disease

Fig. 3: Histological analysis of muscle samples of WT, mdx and nintedanib-treated mdx mice.

From: Nintedanib decreases muscle fibrosis and improves muscle function in a murine model of dystrophinopathy

Fig. 3

Hematoxylin and eosin (H&E) staining of muscle samples obtained (a–i). Representative examples of cross-sectional muscle fibers within quadriceps (a–c), diaphragm (d–f), and tibialis anterior (g–i) of WT, mdx and nintedanib-treated mdx mice. Schematic representation of the fiber mean area in quadriceps (j), diaphragm (k), and tibialis anterior (l) showed no differences on the size of the muscle fibers. Analysis of the proportion of central nucleated fibers in quadriceps (m), diaphragm (n), and tibialis anterior (o) did not present significant differences after 1 month of treatment with nintedanib (60 mg/kg). Data are expressed as means ± SD. Genetic background mouse strain C57BL (WT); n = 5, mdx mice (mdx), n = 5; nintedanib-treated mdx mice (mdx + Ninte), n = 7. Data are expressed as means ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001. Scale bar = 100 µm

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