Fig. 6: RT-qPCR studying mRNA expression of fibrosis-related genes. | Cell Death & Disease

Fig. 6: RT-qPCR studying mRNA expression of fibrosis-related genes.

From: Nintedanib decreases muscle fibrosis and improves muscle function in a murine model of dystrophinopathy

Fig. 6

Collagen type I alpha 1 chain (Col1a1), Collagen type III alpha 1 chain (Col3a1), Fibronectin 1 (Fn1), Platelet-derived growth factor A (Pdgfa), Platelet-derived growth factor B (Pdgfb), Connective tissue growth factor (Ctgf), Transforming growth factor beta 1 (Tgfβ1), and Adhesion G protein-coupled receptor E1 (Adgre1) showed changes in relative abundance following nintedanib in quadriceps (a–h), diaphragm (i–p), and tibialis anterior (q–y). Col1a1, Col3a1, Pdgfa, Tgfb1, and Adgre1 gene expression was increased in mdx mice compared with WT. Col1a1, Col3a1, Pdgfa, Pdgfb, Tgfb1, and Adgre1 expression was reduced in all muscles analyzed from nintedanib-treated mdx mice compared with mdx mice. In contrast, Ctgf expression was increased in muscles from nintedanib-treated mdx mice compared with mdx mice. Data are expressed as means ± SD. Genetic background mouse strain C57BL (WT); n = 5, mdx mice (mdx), n = 5; nintedanib-treated mdx mice (mdx + Ninte), n = 7. *P < 0.05, **P < 0.01, ***P < 0.005

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