Fig. 3: HUC-MSCs significantly downregulated the expression of MCP-1 in β-cells and induced M2 macrophage polarization in pancreatic islets. | Cell Death & Disease

Fig. 3: HUC-MSCs significantly downregulated the expression of MCP-1 in β-cells and induced M2 macrophage polarization in pancreatic islets.

From: Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice

Fig. 3

a Photomicrographs of representative islets stained with anti-insulin (green) and anti-MCP-1 (red) antibodies from the Con, T2D, and the hUC-MSCs group. Percentage of insulin+ cells expressing MCP-1was shown in b. c Serum level of MCP-1 was determined by AimPlex™ assay. d Photomicrographs of representative islets stained with anti-CD11c (green) and anti-Fizz1 (red) antibodies from the Con, T2D, and the hUC-MSCs group. The dotted line circled areas are pancreatic islets. The boxed region showed CD11c and Fizz1 double positive cells within islets, enlarged drawings of which were shown at the bottom of the image. Quantifications of CD11c+ cells and Fizz1+ cells within islets were separately presented in e and f. Ratio of Fizz1+ cells/CD11c+ cells was calculated and presented in g. Scale bar, 50 μm. n = 5 mice per group. The results were presented as the means ± SD. *p < 0.05, **p < 0.01. MCP-1 monocyte chemotactic protein 1

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