Fig. 2: MiR-21 inhibits DAMP-triggered inflammatory cytokine production. | Cell Death & Disease

Fig. 2: MiR-21 inhibits DAMP-triggered inflammatory cytokine production.

From: MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7

Fig. 2

a HMGB1 and HSP60 production in homogenate of heart tissues (left) or sections (right) in WT mice at the indicated days after MI or sham operation was, respectively, detected by ELISA or immunohistochemical staining. Scale bars = 50 μm. b, c ELISA of cytokines in supernatants of peritoneal macrophages isolated from miR-21 knockout (KO) or littermate control WT mice stimulated with rmHMGB1 (1 μg/ml) (b) or rmHSP60 (1 μg/ml) (c) for the indicated times. Data represent the mean ± SEM (n = 6 independent preparations). d, e ELISA of cytokines in supernatants of macrophages 48 h after transfection with miR-21 or control mimics followed by treatment with rmHMGB1 (1 μg/ml) (d) or rmHSP60 (1 μg/ml) (e) for the indicated times. Data represent the mean ± SEM (n = 6 independent preparations). **P < 0.01, ***P < 0.001 (two-way ANOVA)

Back to article page