Fig. 5: Knockdown of KBTBD7 decreases DAMP-triggered inflammatory cytokine production. | Cell Death & Disease

Fig. 5: Knockdown of KBTBD7 decreases DAMP-triggered inflammatory cytokine production.

From: MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7

Fig. 5

a Q-PCR analysis of Kbtbd7 mRNA (normalization to β-actin) or immunoblot analysis of its protein expression in macrophages transfected with control siRNA or Kbtbd7 siRNA. b ELISA of cytokines in supernatants of macrophages 48 h after transfection with control siRNA or Kbtbd7 siRNA followed by treatment with rmHMGB1 (1 μg/ml) or rmHSP60 (1 μg/ml) for the indicated times. c ELISA of cytokines in supernatants of miR-21-deficient or WT macrophages 48 h after transfection with control siRNA or Kbtbd7 siRNA followed by treatment with rmHMGB1 (1 μg/ml) or rmHSP60 (1 μg/ml) for 8 h. Data represent the mean ± SEM (n = 6 independent preparations). **P < 0.01. One-way ANOVA (a) or two-way ANOVA (b, c)

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