Fig. 6: KBTBD7 enhances p38 and NF-κB activation by interacting with MKK3/6. | Cell Death & Disease

Fig. 6: KBTBD7 enhances p38 and NF-κB activation by interacting with MKK3/6.

From: MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7

Fig. 6

a–d Immunoblot analysis of phosphorylation (p-) levels of proteins in lysates of macrophages 48 h after transfection with control siRNA or Kbtbd7 siRNA followed by treatment with rmHSP60 (1 μg/ml) for the indicated times. e, f Immunoblot analysis of phosphorylation (p-) level of MKK3/6 in lysates of heart tissues from sham group or miR-21 KO and WT mice at the indicated times after MI. g Immunoblot analysis of MKK3, MKK6, and KBTBD7 immunoprecipitated with anti-KBTBD7 antibody from lysates of macrophages treated with rmHSP60 (1 μg/ml) for the indicated times. Quantification presented as the relative densitometry intensity of the phosphorylated protein to total protein (b), β-actin (d), or GAPDH (f) in the corresponding same lane. Data represent three individual experiments and the mean ± SD. *P < 0.05, **P < 0.01 vs. Kbtbd7 siRNA (b), control siRNA (d), or KO (f) at the same indicated times (two-way ANOVA)

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