Fig. 4: HIPK2 overexpression increases the autophagic flux in mice with CLP-induced liver injury and inhibits the LPS-induced apoptosis of primary hepatocytes. | Cell Death & Disease

Fig. 4: HIPK2 overexpression increases the autophagic flux in mice with CLP-induced liver injury and inhibits the LPS-induced apoptosis of primary hepatocytes.

From: Overexpression of homeodomain-interacting protein kinase 2 (HIPK2) attenuates sepsis-mediated liver injury by restoring autophagy

Fig. 4

a, b Effects of HIPK2 on levels of the LC3-II and p62 proteins in the liver; the data are presented as means ± SEM and are representative of three separate experiments, #p < 0.05 compared with the control group and + p < 0.05 compared with the CLP plus Ad-vector group. c Effects of Ad-HIPK2 on hepatic LC3-II expression in of the group treated with bafilomycin A (0.2 μM); the data are presented as means ± SEM and are representative of three separate experiments, #p < 0.05 compared with the Ad-vector (control) group at the indicated time point. d Effect of chloroquine (CQ, 50 mg/kg per day) on levels of the LC3-II protein in the liver; the data are presented as means ± SEM and are representative of three separate experiments, #p < 0.05 compared with the sham plus Ad-vector group in the absence of CQ, + p < 0.05 compared with the CLP plus Ad-vector group in the absence of CQ, and @p < 0.05 compared with the CLP plus Ad-HIPK2 group in the absence of CQ. e, f, g Effect of chloroquine (CQ) on serum concentrations of AST, ALT, and ALP in the HIPK2-overexpressing liver tissues, as determined by ELISAs; the data are presented as means ± SEM (n = 30 mice per group), #p < 0.05 compared with the sham plus Ad-vector group in the absence of CQ, + p < 0.05 compared with the CLP plus Ad-vector group in the absence of CQ, and @p < 0.05 compared with the CLP plus Ad-HIPK2 group in the absence of CQ. h Effect of chloroquine (CQ) on the sepsis-induced lethality of HIPK2-overexpressing mice (n = 30 mice per group); + p < 0.05 compared with the CLP plus Ad-vector group in the absence of CQ and @p < 0.05 compared with the CLP plus Ad-HIPK2 group in the absence of CQ. In this section, CLP (n = 30) was performed on the fifth day after the adenovirus injection, and CQ (50 mg/kg) was administered 1 h prior to CLP. i Effect of HIPK2 overexpression on LPS (0.5 μg/mL)-induced apoptosis of primary hepatocytes, as analysed by flow cytometry. j Percentage of apoptotic cells induced by LPS; data are presented as means ± SEM and are representative of three separate experiments, #p < 0.05 compared with the Ad-vector group, + p < 0.05 compared with the LPS plus Ad-vector group

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