Fig. 3: DUSP2 is a target of miR-361-3p. | Cell Death & Disease

Fig. 3: DUSP2 is a target of miR-361-3p.

From: MiR-361-3p regulates ERK1/2-induced EMT via DUSP2 mRNA degradation in pancreatic ductal adenocarcinoma

Fig. 3: DUSP2 is a target of miR-361-3p.The alternative text for this image may have been generated using AI.

a Intersections among four miRNA target prediction algorithms. b Schematic representation of DUSP2 3′ UTR indicating the predicted binding site of miR-361-3p. c–f Relative expression of DUSP2 mRNA and protein after treatment with miR-361-3p mimic or inhibitor. g Schematic diagram of the luciferase reporter plasmids of pMIR-wild type-DUSP2 3′ UTR and pMIR-mutant-DUSP2 3′ UTR. h, i Luciferase activity assays was performed to confirm the direct binding efficiency of miR-361-3p and its putative target DUSP2. j Representative images of immunohistochemistry analyses to detect the DUSP2 protein in orthotopic tumors from negative control and miR-361-3p knockdown group. Data are shown as the mean ± SD of three replicates; *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant

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