Fig. 4: DUSP2 is involved in miR-361-3p-induced EMT and ERK activation. | Cell Death & Disease

Fig. 4: DUSP2 is involved in miR-361-3p-induced EMT and ERK activation.

From: MiR-361-3p regulates ERK1/2-induced EMT via DUSP2 mRNA degradation in pancreatic ductal adenocarcinoma

Fig. 4: DUSP2 is involved in miR-361-3p-induced EMT and ERK activation.The alternative text for this image may have been generated using AI.

a DUSP2 was immunoprecipitated from cell lysates of BxPC-3 and PANC-1 and ERK1/2 was detected using ERK1/2 antibody. b, c PANC-1 and SW1990 cells were transfected with miR-361-3p mimic, DUSP2 plasmid alone, or the combination of miR-361-3p mimic and DUSP2 plasmid. Forty-eight hours after transfection, the cell lysates were subjected to western blot. b, d BxPC-3 and CFPAC-1 were transfected with miR-361-3p inhibitor, si-DUSP2 alone, or the combination of miR-361-3p inhibitor and si-DUSP2. 48 h after transfection, the cell lysates were subjected to western blot. b, e, f The role of DUSP2 in miR-361-3p-induced migration and invasion was demonstrated by Transwell assay in PANC-1 and BxPC-3 cell lines. g Clinical specimens of PDAC with low and high TNM stages were stained for miR-361-3p, DUSP2, p-ERK1/2, Vimentin, E-cadherin, and N-cadherin. Representative images from a tissue microarray are shown. Data are shown as the mean ± SD of three replicates; *P < 0.05; **P < 0.01; ***P < 0.001

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