Fig. 3: Loss of neural progenitors caused by altered Sox2ot is due to early differentiation.

a–d Electroporation of ectopic Sox2ot (OE) at E13.5 for analysis at E17.5 significantly increased the number of Tbr1+ or Satb2+ cells co-labeled with GFP in the cortex, compared to the control (Ctrl). e–h Electroporation of shRNAs against Sox2ot (shSox2ot A and shSox2ot B) at E13.5 for analysis at E17.5 significantly decreased the number of Tbr1+ or Satb2+ cells co-labeled with GFP in the cortex. Yellow and white cells indicate co-labeled cells. Data are presented as mean ± SD; n ≥ 5 sections from at least four different brains; p values in relation to the empty vector control (***p < 0.001). Scale bars: 50 µm