Fig. 4: Sox2ot is localized to the nucleus in neuroectodermal cells and neural progenitors. | Cell Death & Disease

Fig. 4: Sox2ot is localized to the nucleus in neuroectodermal cells and neural progenitors.

From: Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2

Fig. 4

a Diagram of the mouse embryonic stem (ES) cell culture protocol for neuroectoderm differentiation. b, c Real-time RT-PCR detecting the relative expression of markers for stem cells (b) and neuroectoderms (c) in JQ1 (gray) and DMSO- (black) treated ES cells. d Real-time RT-PCR detecting Sox2 and Sox2ot in JQ1 and DMSO-treated ES cells. e, f Real-time RT-PCR detecting Sox2ot, Gapdh, and Xist expression in nuclear (N, black) and cytoplasmic (C, gray) fractions of neuroectodermal cells differentiated from female mouse LF2 ES cells. g Nuclear expression of Sox2ot in cultured mouse neural progenitors derived from E12.5 cortex detected by RNA fluorescent in situ hybridization (FISH). Data are presented as mean±SD; n = 3 for all real-time RT-PCR; p values in relation to the control (**p < 0.01, ***p < 0.001). Scale bars=20 µm

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