Fig. 5: Sox2ot binds YY1 in an RNA immunoprecipitation (RIP) assay. | Cell Death & Disease

Fig. 5: Sox2ot binds YY1 in an RNA immunoprecipitation (RIP) assay.

From: Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2

Fig. 5

a Schematic summary of the RIP protocol. b RIP with YY1, Pax6, or control antibody. Relative Sox2ot enrichment detected by real-time RT-PCR. c Real-time RT-PCR detection of various brain mRNAs and lncRNAs in YY1 RIP RNA. d Real-time RT-PCR detection of positive control Paupar lncRNA in Pax6 RIP RNA. Data are presented as mean± SD; n = 3 individual experiments for all real-time RT-PCR; p values in relation to the control (***p < 0.001)

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