Fig. 3: DNA methyltransferases expression and global genomic DNA methylation profile in myocardium tissues.

DNMT1 (a), DNMT3b (b), DNMT3a (c) and DNMT3L (d) expression was shown in the myocardium tissues of prenatally DEX-exposed offspring and prenatally NS-exposed offspring. Representative immunoblots were shown in the upper panel, corresponding to each densitometric scan normalized to GAPDH as an internal standard, which were shown in the lower panel. Global DNA methylation increased in DEX-exposed adult male offspring’s myocardium compared with that from NS-exposed adult male offspring (e). Data shown are mean ± SEM. ***p < 0.001, ****p < 0.0001