Fig. 4: Alcohol treatment promotes PIASy expression in Huh7 cells.
From: Alcohol-induced autophagy via upregulation of PIASy promotes HCV replication in human hepatoma cells
a The effect of alcohol on mRNA expression of four members (PIAS1, PIASx(a,b), PIAS3, and PIASy). Huh7 cells were treated with or without alcohol at indicated concentrations for 96 h. The mRNA levels of four types of PIASs, with normalization to the corresponding GAPDH mRNA level, are expressed as the fold of control (without alcohol treatment, which was defined as 1). b A representative western blot image shows the protein levels of PIASy in alcohol-treated or alcohol-untreated cells. The relative ratios of PIASy/β-actin were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). c, d Time-course effect of alcohol on PIASy expression. Huh7 cells were treated with alcohol (80 mM) for different times (0, 48, 96, and 144 h). c The mRNA levels of PIASy, with normalization to the corresponding GAPDH mRNA level, are expressed as the fold of control (without alcohol treatment, which was defined as 1). d A representative western blot image shows the protein levels of PIASy. The relative PIASy/β-actin ratios were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). The data are the mean ± SD of the results of three independent experiments. *p < 0.05, **p < 0.01
