Fig. 5: PIASy is involved in alcohol-enhanced HCV replication and alcohol-induced autophagy.
From: Alcohol-induced autophagy via upregulation of PIASy promotes HCV replication in human hepatoma cells
a, b The effect of overexpression of PIASy on alcohol-enhanced HCV replication and alcohol-induced autophagy. a HCV JFH-1-infected Huh 7 cells were transfected with pCMV-myc-PIASy or empty vector. At 12 h post transfection, the cells were treated with or without alcohol (80 mM) for 72 h. The cellular proteins were extracted for western blot assay. The relative ratios of PIASy/β-actin and HCV core/β-actin were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). b Huh 7 cells were transfected with pCMV-myc-PIASy or empty vector. At 12 h post transfection, the cells were treated with or without alcohol (80 mM) for 72 h. The cellular proteins were extracted for western blot assay. The relative ratios of PIASy/β-actin, LC3B-II/β-actin, LC3B-II/LC3B-I, and p62/β-actin were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). c, d The effect of silencing expression of PIASy on alcohol-enhanced HCV replication and alcohol-induced autophagy. c HCV JFH-1-infected Huh 7 cells were transfected with shRNA-PIASy or control-scrambled shRNA. At 12 h post transfection, the cells were treated with or without alcohol (80 mM) for 72 h. The cellular proteins were extracted for western blot assay. The relative ratios of PIASy/β-actin and HCV core/β-actin were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). d Huh 7 cells were transfected with shRNA-PIASy or control-scrambled shRNA. At 12 h post transfection, the cells were treated with or without alcohol (80 mM) for 72 h. The cellular proteins were extracted for western blot assay. The relative ratios of PIASy/β-actin, LC3B-II/β-actin, LC3B-II/LC3B-I, and p62/β-actin were calculated and shown as the fold of control (without alcohol treatment, which was defined as 1). The data are the mean ± SD of the results of three independent experiments. *p < 0.05, **p < 0.01
