Fig. 8: PIASy induces autophagy via regulation of SUMO1 pathway in Huh7 cells.
From: Alcohol-induced autophagy via upregulation of PIASy promotes HCV replication in human hepatoma cells
The effect of overexpression (a) or silencing expression (b) of PIASy on levels of SUMO-conjugated proteins and autophagy-related genes. Huh7 cells were transfected with pCMV-myc-PIASy/empty vector (a) or shRNA-PIASy/control- scrambled shRNA (b) for 96 h. The western blot images show the protein levels of PIASy, SUMO1-conjugated proteins, SUMO2/3-conjugated proteins, Atg7, and Atg5–12 in PIASy-overexpressed (a) or PIASy-downexpressed (b) cells. The relative ratios of PIASy/β-actin, Atg7/β-actin, and Atg5–12/LC3B-I were calculated and shown as the fold of control (with empty vector transfection (a) or with scrambled shRNA transfection (b), which was defined as 1, respectively). The data are the mean ± SD of the results of three independent experiments. *p < 0.05, **p < 0.01
