Fig. 1: The growth inhibitory effects of GDC-0941 for 4T1 tumor cells in in vitro and in vivo assays were not consistant. | Cell Death & Disease

Fig. 1: The growth inhibitory effects of GDC-0941 for 4T1 tumor cells in in vitro and in vivo assays were not consistant.

From: Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling

Fig. 1

a Clonogenic proliferation assay of 4T1 cells treated with 1 µM GDC-0941 or vehicle for 10 days. Means ± S.D. of three independent experiments are shown. b Tumor volumes in 4T1 allograft-bearing mice following treatment with GDC-0941 (125 mg/kg/day, oral gavage, n = 5) or vehicle control (n = 5) for 14 days. Error bars represent mean ± S.E.M. c Western blot analysis of proteins as indicated in 4T1 cells treated with 1 µM GDC-0941 or vehicle for 24 h. Vinculin was used as a loading control. d Representative images of immunohistochemical staining for p-AKT in tumors from mice bearing 4T1 allografts following treatment with GDC-0941 (n = 5) or vehicle control (n = 5) for 3 days. Scale bar, 50 μm. Quantification of the IOD value is shown. Error bars represent mean ± S.E.M. **P < 0.01 (Student’s t-test)

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