Fig. 3: NF-κB signaling contributed to compromised efficacy of GDC-0941 in 4T1 cells in the presence of macrophages. | Cell Death & Disease

Fig. 3: NF-κB signaling contributed to compromised efficacy of GDC-0941 in 4T1 cells in the presence of macrophages.

From: Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling

Fig. 3

a Clonogenic proliferation assay of 4T1 cells treated with 1 µM GDC-0941 or DMSO vehicle for 10 days. 4T1 cells were cultured alone or co-cultured with RAW264.7 or BMM macrophages as indicated. All experiments were performed in triplicate and representative images of plates are shown. Error bars represent mean ± S.D. b Western blot analysis of proteins as indicated in 4T1 cells treated with 1 µM GDC-0941 or vehicle for 24 h. 4T1 cells were cultured alone or co-cultured with RAW264.7 macrophages. Vinculin was used as a loading control. c ELISA analysis of TNFα in culture media from co-cultured 4T1 and RAW264.7 cells treated with 1 µM GDC-0941 or vehicle for 24 h. Means ± S.D. of three independent experiments are shown. d Representative images of immunofluorescent staining of p65 cellular localization in 4T1 cells treated with 1 µM GDC-0941 or vehicle for 24 h. 4T1 cells were cultured alone or co-cultured with RAW264.7 macrophages. Representative images of three independent experiments are shown. *P < 0.05; ***P < 0.001 (Student’s t-test)

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