Fig. 6: Combined use of GDC-0941 and Aspirin significantly inhibited the NF-κB signaling and macrophages infiltration in vivo. | Cell Death & Disease

Fig. 6: Combined use of GDC-0941 and Aspirin significantly inhibited the NF-κB signaling and macrophages infiltration in vivo.

From: Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling

Fig. 6

a Western blot analysis of proteins as indicated in 4T1 tumors of Balb/c mice following treatment with GDC-0941 (125 mg/kg/day, oral gavage) and Aspirin (100 mg/kg/day, oral gavage) as single agents or in combination (n = 5 per treatment group) for 3 days. Vinculin was used as a loading control. b Representative images of immunohistochemical staining for p65 and CD68 of 4T1 tumors after the mice were treated with GDC-0941 and Aspirin as single agents or in combination (n = 5 per treatment group) for 3 and 10 days, respectively. Scale bar, 50 μm. Percentages of CD68 staining positive cells and nuclear p65 staining positive cells are shown. Error bars represent mean ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)

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