Fig. 1: miR-205-5p inhibition impairs BCSCs proliferation in vitro.
From: MiR-205-5p inhibition by locked nucleic acids impairs metastatic potential of breast cancer cells
a Cell proliferation assay, by cell count of Trypan blue-stained cells, of BCSC#1 and BCSC#3 infected with mir-205-5p silencing lentivector (shmiR-205) or with the empty vector (ctr). Data are presented as mean ± SD (ANOVA analysis) of different experiments (n = 2) each performed in triplicate. b qRT-PCR of miR205-5p expression levels of BCSCs#3 treated with 40 pmol (left) or 120 pmol (right) of miRCURY LNA miR-205-5p inhibitor (LNA-i-miR-205-5p) or the scrambled control (scr) for 24, 48 and 72 h. Data are presented as mean ± SD (T-test) of three independent experiments each performed in triplicate. c Cell proliferation assay (cell count) of BCSCs#3 treated every 72 h with 40 pmol of LNA miR-205-5p inhibitor or a scramble control and cultured up to 7 days. Data are presented as mean ± SD (ANOVA test) of two independent experiments each performed in duplicate. d qRT-PCR of miR-205-5p levels of BCSCs treated as described above after 7 days of in vitro culture. Data are presented as mean ± SD (T-test) of two independent experiments each performed in triplicate
