Fig. 5: miR-205-5p regulates invasion ability of BCSCs in vitro and in vivo.
From: MiR-205-5p inhibition by locked nucleic acids impairs metastatic potential of breast cancer cells
a Representative images (upper) of transwell invasion assay (24 h) of BCSCs#3 infected with miR-205-5p silencing lentivector (shmiR-205-5p) or with the control vector (ctr) stained with crystal violet. Quantitative data are presented as mean ± SD of percentage of invasion of three independent experiments (lower). b Western blot analysis of Vimentin and Twist protein levels of BCSCs#3 infected with miR-205-5p silencing lentivector or with the control vector. Actin was used as a loading control (upper panel). qRT-PCR of Twist and Vimentin mRNA expression levels of BCSCs#3 infected as described above. Data presented as mean ± SD (T-test analysis) of three independent experiments (lower panel). c Representative H&E staining (upper) of lung metastases of NSG mice (6 per group) after tail vein injection of 2 × 105 BCSCs #3 infected with miR-205-5p silencing lentivector or with the control vector (scale bar: 400 μm). Mice were monitored up to 35 days and then sacrificed. Graphs show, respectively, the number (bottom left) and the size (bottom right) of lung metastases for each group. Data were analyzed with T-test. d Representative immunohistochemical staining of Ki67 in lung metastases of NSG mice treated as described above (scale bar: 200 μm). Percentage of Ki67-positive cells are shown in the graph (bottom) analyzed with T-test
