Table 1 Primer sequences used for real-time PCR for human samples

From: Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts

Gene

Sequence (sense, antisense: 5′–3′)

MAP1LC3B

AGCAGCATCCAACCAAAATC

CCGTTCACCAACAGGAAGAA

BECN1

TCCACAGAAAGTGCCAACAG

GTCAAAAAGGTCCCCAGTGA

ATG5

GCCATCAATCGGAAACTCAT

CAGCCACAGGACGAAACAG

SQSTM1(p62)

TAGGAACCCGCTACAAGTGC

GAGAAGCCCTCAGACAGGTG

RANKL

GATGAAAGGAGGAAGCACCA

TAAGGAGGGGTTGGAGACCT

RANK

GCCTTTCTCTTGGCATCATC

TGCTTTTCCCCAGTATTTGC

OPG

CAGGCACTTGAGGCTTTCA

TTGGGGTTTATTGGAGGAGA

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