Fig. 1: Optimization of 3D human embryonic stem cell (hESC) suspension culture system.

a Comparison of the average folds of hESC proliferation suspended in different medium. b Morphology of hESC spheres with different initial cell seeding density. Scale bar, 100 μm. c Comparison of the average folds of hESC proliferation suspended in E8 medium with different initial cell seeding density. d Cell viability of spheres suspended in E8 medium with different initial cell seeding density