Fig. 4: Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. | Cell Death & Disease

Fig. 4: Comparison of MSCs derived from 2D-hESCs and 3D-hESCs.

From: A fully defined static suspension culture system for large-scale human embryonic stem cell production

Fig. 4

a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, CD29, and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)

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