Fig. 2: HSV1 (FmutRHIM) virus attenuation in vitro or in vivo is reversed when ZBP1 is absent. | Cell Death & Disease

Fig. 2: HSV1 (FmutRHIM) virus attenuation in vitro or in vivo is reversed when ZBP1 is absent.

From: Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1

Fig. 2

a, b Viral titers of WT MEF and Zbp1−/− MEF cells infected with HSV1(FmutRHIM) viruses at (a) MOI = 5 or (b) MOI = 0.1 for indicated times. Cells together with the supernatants were harvested and titered by a standard viral plaque assay. pfu plaque-forming units. c Viral titers of spleen of WT, Zbp1−/−, Ripk3−/−, and Mlkl−/− mice infected with 1 × 107 pfu HSV1 (FmutRHIM) per mouse via intraperitoneal inoculation (i.p.). One-way ANOVA multiple comparison post-test analyses were conducted using GraphPad Prism 5. p < 0.05 was considered significant

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