Fig. 4: HSV1 (FmutRHIM)-induced necroptosis requires nascent transcription.

a Cell viability of SVEC4-10 cells infected with HSV1 (FmutRHIM) (UV (−)) or UV-irradiated FmutRHIM virus (UV (+)). Data with error bars depict mean ± standard deviation (SD); with significance ***p < 0.001 indicated above bars. b IB of VP16 and β-actin in SVEC4-10 cells infected by FmutRHIM or UV-irradiated virus at 4 and 16 hpi. c, d Cell viability of SVEC4-10 cells infected with HSV1 (FmutRHIM) as well as treated with actinomycin D (ActD) (c) or phosphonoformate (PFA) (d) at the indicated times post infection