Fig. 2: NS1619 and DHEA activate NRF2. | Cell Death & Disease

Fig. 2: NS1619 and DHEA activate NRF2.

From: Selective killing of human T-ALL cells: an integrated approach targeting redox homeostasis and the OMA1/OPA1 axis

Fig. 2

A Immunofluorescence of TALL-1 cells following 24 h of treatment with NS1619 and DHEA. An anti-phosphoS40-NRF2 antibody was used to detect pNRF2 (green). Propidium iodide (PI, red) and APC-conjugated anti-CD7 antibody (blue) were used as a nuclear and membrane markers, respectively. Lower panels show the pixel dot plots of the pNRF2 and PI signals in representative fields. B qRT-PCR of NRF2 target genes NQO1 (NAD(P) H:quinone oxidoreductase 1), G6PD (glucose-6-phosphate dehydrogenase), and FHT1 (ferritin heavy chain 1) after 24 h of treatment of TALL-1 cells with NS1619 and DHEA. β2-microglobulin (B2M) was used as a housekeeping mRNA and all values were scaled against untreated cells (CTR). Mean values and SE bars from seven experiments are shown

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