Fig. 4: Effects of NS1619 and DHEA on OPA1. | Cell Death & Disease

Fig. 4: Effects of NS1619 and DHEA on OPA1.

From: Selective killing of human T-ALL cells: an integrated approach targeting redox homeostasis and the OMA1/OPA1 axis

Fig. 4

A Immunoblot of a representative experiment showing the five major OPA1 isoforms (A–E) in TALL-1 cells after 24 h of the indicated treatments. Cleaved OPA1 ratios (see Materials and Methods) are shown below the blots. NAC N-acetyl-l-cysteine. B Quantitative RT-PCR analysis of OMA1 mRNA expression in TALL-1 cells after electroporation with siRNA specific for OMA1 (siOMA1) or a control siRNA(siCTR). β2-microglobulin (B2M) was used as a housekeeping mRNA and values were scaled against the CTR. Mean values and SE bars from three experiments are shown. C Immunoblots of a representative experiment showing the five major OPA1 isoforms in TALL-1 cells transfected with control siRNA (left) or OMA1 siRNA (right) after 24 h of the indicated treatments. Cleaved OPA1 ratios (see Materials and Methods) are shown below the blots. D Specific cell death of TALL-1 cells after electroporation with control siRNA (continuous lines) or OMA1-specific siRNA (dashed lines) followed by treatment with NS1619 (red), DHEA (green) or NS1619 + DHEA (blue). Mean values of specific cell death and SE bars from three independent experiments are shown

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