Fig. 1: TRMP is up-regulated by p53.

a H1299 cells with doxycycline-inducible expression of wild-type p53 (H1299-Tet-On-p53) were incubated with doxycycline (1 μg/ml) for the indicated periods of time. Total RNA was then subjected to real-time RT-PCR analysis to examine expression levels of the indicated 10 long non-coding RNAs (lncRNAs). The detailed information of these lncRNAs is shown in Supplementary Table S1. Data shown are mean ± SD of three independent experiments. Cell lysates were also analyzed by western blotting with the indicated antibodies. b H1299 cells were infected with lentiviruses expressing wild-type p53. Forty-eight hours later, total RNA was subjected to real-time RT-PCR analysis to examine TRMP levels. Data shown are mean ± SD of three independent experiments. Cell lysates were also analyzed by western blotting. c A549, U2OS, and HCT116 cells were infected with lentiviruses expressing either control shRNA or p53 shRNA. Forty-eight hours later, total RNA was subjected to real-time RT-PCR analysis to examine TRMP levels. Data shown are mean ± SD of three independent experiments. Cell lysates were also analyzed by western blotting with the indicated antibodies. d A549 cells were infected with lentiviruses expressing either control shRNA or p53 shRNA. Forty-eight hours, cells were treated with doxorubicin (0.5 μg/ml) for the indicated periods of time. Total RNA was then subjected to real-time RT-PCR analysis to examine TRMP levels. Data shown are mean ± SD of three independent experiments. Cell lysates were also analyzed by western blotting