Fig. 1: BEZ235 suppressed PI3K/AKT/mTOR signaling and cell viability.

a T47D and b BT474 cells were treated with DMSO or different concentrations of BEZ235 for 24 h. Whole-cell lysates were prepared, and western blot analyses were performed to detect p-p70S6K, p70S6K, AKT, p-AKT (S473), and p-AKT (T308). c T47D and d BT474 cells were exposed to increasing doses of BEZ235 for 24 h. Cell viability was evaluated by MTT assay. **P < 0.01 versus control group. Data shown are mean ± SEM of at least three independent experiments