Fig. 2: Deletion of Ripk3 or/and Mlkl genes reduced renal fibrosis after IRI.
From: RIPK3-MLKL-mediated necroinflammation contributes to AKI progression to CKD
All mice were subjected to IRI with 40-min ischemia and then were fed with normal rodent chow for the indicated time. a Kidney morphology by Masson’s trichrome staining. Representative micrographs in kidney sections. Bar = 100 μM. b Renal fibrosis scores obtained from Masson’s trichrome-stained sections were analyzed with Image J. n = 6. ##P < 0.01 vs. WT sham group; **P < 0.01 vs. WT IRI. c Representative immunoblot for RIPK3, MLKL, p-Smad3, α-SMA, Col I, and β-actin by western blot in total kidney lysates. n = 6
