Fig. 4: Constitutive hTERT expression is required for cell cycle progression of WS cell-derived iPSCs.

a hTERT expression in WS-ihTERT and WT-ihTERT iPSCs at passage 10 (P10). b BrdU incorporation in WS and WT iPSCs with inducible hTERT with or without Dox. c Cell cycle analysis at different time points after synchronization with nocodozal. d, e Percentages of cells at S phase after synchronization with nocodozal. WS-ihTERT-, Werner syndrome iPSCs (AG03141) with inducible hTERT without Dox treatment; WT-ihTERT-, Wild-type iPSCs (AG10803) with inducible hTERT without Dox treatment